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嗜热脂肪芽孢杆菌ATCC12980中的一个新型蔗糖-果聚糖酶基因簇。

A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980.

作者信息

Li Y, Triccas J A, Ferenci T

机构信息

Department of Microbiology G08, University of Sydney, NSW, Australia.

出版信息

Biochim Biophys Acta. 1997 Sep 12;1353(3):203-8. doi: 10.1016/s0167-4781(97)00103-6.

DOI:10.1016/s0167-4781(97)00103-6
PMID:9349714
Abstract

A 3.1 kb fragment of Bacillus stearothermophilus ATCC12980 DNA permitted growth of Escherichia coli on sucrose. The fragment encoded genes for a levansucrase (surB) and also a levanase (surC). SurB and SurC shared 97% and 43% amino acid identity with the corresponding SacB and SacC proteins of Bacillus subtilis, whose sacB and sacC genes are organised very differently.

摘要

嗜热脂肪芽孢杆菌ATCC12980的一段3.1 kb DNA片段使大肠杆菌能够在蔗糖上生长。该片段编码一种果聚糖蔗糖酶(surB)和一种果聚糖酶(surC)的基因。SurB和SurC与枯草芽孢杆菌相应的SacB和SacC蛋白分别具有97%和43%的氨基酸同一性,而枯草芽孢杆菌的sacB和sacC基因的组织方式非常不同。

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