Amonsin A, Wellehan J F, Li L L, Vandamme P, Lindeman C, Edman M, Robinson R A, Kapur V
Department of Clinical and Population Science, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
J Clin Microbiol. 1997 Nov;35(11):2894-8. doi: 10.1128/jcm.35.11.2894-2898.1997.
Ornithobacterium rhinotracheale is a recently described gram-negative rod-shaped bacterium associated with respiratory tract infections in poultry. In order to determine the molecular epidemiology of this bacterium, we characterized 55 O. rhinotracheale isolates from eight countries on four continents by multilocus enzyme electrophoresis (MLEE), repetitive sequence based-PCR (rep-PCR), and 16S rRNA gene sequencing. MLEE discriminated the O. rhinotracheale isolates into six electrophoretic types (ETs), of which only three ETs were recovered from domesticated poultry. The 16S rRNA gene sequence and rep-PCR analyses confirmed the results obtained by MLEE and indicated limited heterogeneity among isolates of O. rhinotracheale recovered from poultry. Taken together, the results of our analysis demonstrate that the majority of O. rhinotracheale isolates recovered from domesticated poultry throughout the world are represented by a small group of closely related clones and suggest that the bacterium was recently introduced to domesticated poultry from wild bird populations.
禽源嗜气管鸟杆菌是一种最近才被描述的革兰氏阴性杆状细菌，与家禽呼吸道感染有关。为了确定这种细菌的分子流行病学，我们通过多位点酶电泳（MLEE）、基于重复序列的聚合酶链反应（rep-PCR）和16S rRNA基因测序，对来自四大洲八个国家的55株禽源嗜气管鸟杆菌分离株进行了特征分析。MLEE将禽源嗜气管鸟杆菌分离株分为六种电泳类型（ETs），其中只有三种ETs是从家养家禽中分离得到的。16S rRNA基因序列和rep-PCR分析证实了MLEE获得的结果，并表明从家禽中分离得到的禽源嗜气管鸟杆菌分离株之间的异质性有限。综合来看，我们的分析结果表明，从世界各地家养家禽中分离得到 的大多数禽源嗜气管鸟杆菌分离株都由一小群密切相关的克隆代表，并表明该细菌最近是从野生鸟类种群引入到家养家禽中的。
