Zhang Z, Andrews G A, Chard-Bergstrom C, Minocha H C, Kapil S
Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
J Clin Microbiol. 1997 Nov;35(11):2964-5. doi: 10.1128/jcm.35.11.2964-2965.1997.
A monoclonal antibody (MAb) (Z3A5) against spike protein subunit of bovine coronavirus (BCV) reacted with the virus in formalin-fixed intestines in an immunoperoxidase test. We found an 88% correlation between immunohistochemistry with Z3A5 and in situ hybridization with a BCV nucleoprotein cDNA probe. MAb Z3A5 reacted with 90 BCV isolates from the United States and was an effective reagent for the diagnosis of BCV.
一种针对牛冠状病毒（BCV）刺突蛋白亚基的单克隆抗体（MAb）（Z3A5），在免疫过氧化物酶试验中能与福尔马林固定肠道中的该病毒发生反应。我们发现，用Z3A5进行免疫组织化学检测与用BCV核蛋白cDNA探针进行原位杂交之间存在88%的相关性。单克隆抗体Z3A5与来自美国的90株BCV分离株发生反应，是诊断BCV的有效试剂。
