牛冠状病毒抗原检测酶联免疫吸附测定的开发与应用
Development and applications of a bovine coronavirus antigen detection enzyme-linked immunosorbent assay.
作者信息
Schoenthaler S L, Kapil S
机构信息
Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506-5606, USA.
出版信息
Clin Diagn Lab Immunol. 1999 Jan;6(1):130-2. doi: 10.1128/CDLI.6.1.130-132.1999.
Abstract
We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 10(4) bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.
摘要
我们开发了一种基于单克隆抗体的抗原捕获夹心酶联免疫吸附测定（ELISA）方法用于检测牛冠状病毒。我们将该ELISA方法与电子显微镜检查和血凝试验进行了比较，发现它们之间具有密切的相关性。ELISA的灵敏度为每毫升10%粪便悬液中有10⁴个牛冠状病毒颗粒。与电子显微镜检查相比，牛冠状病毒ELISA的特异性为96%。
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