Lartey R, Ghoshroy S, Ho J, Citovsky V
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794-5215, USA.
Plant J. 1997 Sep;12(3):537-45. doi: 10.1046/j.1365-313x.1997.00537.x.
Tobamoviruses represent a well-characterized system used to examine viral infection, whereas Arabidopsis is a choice plant for most genetic experiments. It would be useful to combine both approaches into one experimental system for virus-plant interaction. Most tobamoviruses, however, are not pathogenic in Arabidopsis. Here, we describe infection of Arabidopsis by a recently discovered crucifer-infecting turnip vein clearing tobamovirus (TVCV). Using this system, we determined patterns and kinetics of viral local and systemic movement within Arabidopsis plants. Localization studies showed that the virus infects both vegetative and reproductive plant tissues. However, there may be a transport barrier between the seed coat and the embryo which virions cannot cross, preventing seed transmission of TVCV. The ability to move both locally and systemically in Arabidopsis, causing mild and fast-developing symptoms but allowing survival and fertility of the infected plants, distinguish TVCV infection of Arabidopsis as a model system to study virus-plant interaction.
烟草花叶病毒属病毒代表了一个用于研究病毒感染的特征明确的系统，而拟南芥是大多数遗传实验的首选植物。将这两种方法结合到一个用于病毒-植物相互作用的实验系统中将会很有用。然而，大多数烟草花叶病毒属病毒对拟南芥没有致病性。在此，我们描述了一种最近发现的感染十字花科植物的芜菁脉明烟草花叶病毒（TVCV）对拟南芥的感染情况。利用这个系统，我们确定了病毒在拟南芥植株内局部和系统移动的模式及动力学。定位研究表明，该病毒感染植物的营养组织和生殖组织。然而，种皮和胚之间可能存在病毒粒子无法跨越的运输屏障，从而阻止了TVCV的种子传播。TVCV在拟南芥中既能局部移动又能系统移动，会引发轻微且快速发展的症状，但能使受感染植株存活并保持育性，这使得TVCV感染拟南芥成为研究病毒-植物相互作用的一个模型系统。
