Steingrímsdóttir T, Ericsson A, Franck A, Waldenström A, Ulmsten U, Ronquist G
Department of Obstetrics and Gynecology, Uppsala University Hospital, Sweden.
Eur J Clin Invest. 1997 Sep;27(9):743-9. doi: 10.1046/j.1365-2362.1997.1810726.x.
The purpose of the study was to investigate by in vitro and in vivo methods the phosphocreatine (PCr)/ ATP ratio as an expression of the energy metabolic state of human myometrium in comparison with striated skeletal muscle. The contents of PCr and adenylates in biopsies of uterine smooth muscle and m. rectus abdominis from seven term pregnant women were determined in vitro and compared with results obtained in vivo by phosphorus magnetic resonance spectroscopy (31P-MRS) in the uterus and m. gastrocnemius of eight non-pregnant women. The PCr/ATP ratio in the striated skeletal muscle was about three times higher than that of the myometrium. The results of the in vitro biopsy part of the study and the in vivo 31P-MRS part conformed with each other. In the biopsies both PCr and ATP concentrations were significantly lower in the myometrium than in the rectus muscle, but the difference for PCr was more pronounced, accounting for the significantly lower PCr/ATP ratio in the uterine smooth muscle. The energy metabolic pattern of uterine smooth muscle differs from that of striated skeletal muscle regarding the contents of high-energy phosphocompounds and the PCr/ATP ratio. This in vivo finding is the first report on human smooth muscle using 31P-MRS.
本研究的目的是通过体外和体内方法，研究磷酸肌酸（PCr）/三磷酸腺苷（ATP）比值，以作为人类子宫肌层与横纹肌相比的能量代谢状态的一种表达。测定了7名足月孕妇子宫平滑肌和腹直肌活检组织中PCr和腺苷酸的含量，并将体外测定结果与8名非孕妇子宫和腓肠肌中通过磷磁共振波谱法（31P-MRS）获得的体内结果进行比较。横纹肌中的PCr/ATP比值约比子宫肌层高3倍。该研究的体外活检部分结果与体内31P-MRS部分结果相互吻合。在活检组织中，子宫肌层的PCr和ATP浓度均显著低于腹直肌，但PCr的差异更为明显，这导致子宫平滑肌中的PCr/ATP比值显著较低。就高能磷酸化合物的含量和PCr/ATP比值而言，子宫平滑肌的能量代谢模式不同于横纹肌。这一体内研究结果是使用31P-MRS对人类平滑肌的首次报道。
