Hollins P J, Nathan M M
Clin Exp Immunol. 1979 Oct;38(1):127-34.
Insulins of differing species, together with chemically modified insulins, were used in cross-reactivity experiments employing selected antisera raised to ox insulin in the Harley guinea-pig. The immunogen had been administered as a water-in-oil emulsion, using H. pertussis vaccine as adjuvant. Antibody was generated by determinants in the C-terminus of the B chain plus the adjacent N-terminus of the A chain, in the central core of the A chain (A8-A14 region) and in its anti-parallel N-terminus of the B chain. From this antibody pool chemically modified ox insulin selected antibody to unaltered determinants. The immunochemical data were compatible with monomeric ox insulin being immunogenic, the immunogen perhaps being recognized by the immune system in the form of the Molecule-II rather than the Molecule-I of the dimer pair (as originally suggested by X-ray crystallographic data).
不同物种的胰岛素以及化学修饰的胰岛素，被用于交叉反应实验，该实验采用在哈雷豚鼠中针对牛胰岛素产生的特定抗血清。免疫原是以油包水乳液的形式给药的，使用百日咳疫苗作为佐剂。抗体由B链C末端加上A链相邻的N末端、A链中央核心（A8 - A14区域）及其B链反平行N末端的决定簇产生。从这个抗体库中，化学修饰的牛胰岛素选择了针对未改变决定簇的抗体。免疫化学数据与单体牛胰岛素具有免疫原性相一致，免疫原可能以分子 - II而非二聚体对中的分子 - I的形式被免疫系统识别（如最初X射线晶体学数据所表明的那样）。
