Hollins P J, Nathan M M
Clin Exp Immunol. 1978 Mar;31(3):464-71.
Female Hartley guinea-pigs were immunized with N-phthaloylated ox insulin (96% NA1,NB1,NB29-triphthaloyl ox insulin) using H. pertussis as adjuvant. Haptenic antibody, which was found in antisera taken 20 days after secondary immunization, was assessed for cross-reaction with a series of N-acylated ox insulins. Cross-reaction readily occurred between haptenic antibody and the partial hapten-bearing antigens, NA1-monophthaloyl and NB1-monophthaloyl ox insulins. The immunochemical data suggested that the NA1-glycyl hapten and the NB1-phenylalanyl hapten were both acting as immune determinants for B-lymphocytes, and the determinant competition was taking place between contralateral facets of the principal immunogen, NA1,NB1,NB29-triphthaloyl ox insulin. The data was also consistent with the view that the overall tertiary structure of N-acylated insulins may be very similar to that of their native insulin, the addition of hapten groups having produced only localized areas of structural perturbation.
以百日咳杆菌作为佐剂，用N-邻苯二甲酰化牛胰岛素（96%的NA1、NB1、NB29-三邻苯二甲酰化牛胰岛素）对雌性哈特利豚鼠进行免疫。在二次免疫后20天采集的抗血清中发现了半抗原抗体，并对其与一系列N-酰化牛胰岛素的交叉反应进行了评估。半抗原抗体与部分带有半抗原的抗原，即NA1-单邻苯二甲酰化和NB1-单邻苯二甲酰化牛胰岛素之间很容易发生交叉反应。免疫化学数据表明，NA1-甘氨酰半抗原和NB1-苯丙氨酰半抗原均作为B淋巴细胞的免疫决定簇，且决定簇竞争发生在主要免疫原NA1、NB1、NB29-三邻苯二甲酰化牛胰岛素的对侧面上。该数据也与以下观点一致，即N-酰化胰岛素的整体三级结构可能与其天然胰岛素非常相似，半抗原基团的添加仅产生了局部结构扰动区域。
