Hollins P J, Smith G H, Himsworth R L
Clin Exp Immunol. 1976 Jun;24(3):507-14.
Subgroups of female Hartley guinea-pigs were immunized with N-carbamylated ox insulin, N-maleylated ox insulin, N-phthaloylated ox insulin, or with the crystalline ox insulin from which the N-acylated insulins had been prepared. The immunogens were administered in water-in-oil emulsions containing pertussis vaccine as adjuvant. Sera obtained 20 days after secondary immunization were assayed for their antibody titres to iodo-ox insulin and their insulin-binding capacities. The data were log transformed for statistical comparison. N-carbamylated ox insulin seemed to be as immunogenic as crystalline ox insulin and no specific carbamyl hapten antibody could be found. N-maleylated and N-phthaloylated ox insulins yelded significantly less antibody cross-reactingwith iodo-ox insulin, but produced a complementary quantity of specific maleyl and phthaloyl hapten antibody respectively. Thus it was shown that in the system used the immune response was partitioned between different determinants, ox insulin and its N-acylated derivatives being equipotent immunogens.
将雌性Hartley豚鼠分成若干亚组，分别用N-氨甲酰化牛胰岛素、N-马来酰化牛胰岛素、N-邻苯二甲酰化牛胰岛素，或用制备N-酰化胰岛素所用的结晶牛胰岛素进行免疫。免疫原在含有百日咳疫苗作为佐剂的油包水乳液中给药。二次免疫20天后获得的血清检测其对碘代牛胰岛素的抗体滴度及其胰岛素结合能力。对数据进行对数转换以进行统计比较。N-氨甲酰化牛胰岛素似乎与结晶牛胰岛素具有相同的免疫原性，且未发现特异性氨甲酰半抗原抗体。N-马来酰化和N-邻苯二甲酰化牛胰岛素产生的与碘代牛胰岛素交叉反应的抗体明显较少，但分别产生了相应数量的特异性马来酰和邻苯二甲酰半抗原抗体。因此表明，在所使用的系统中，免疫反应在不同的决定簇之间进行分配，牛胰岛素及其N-酰化衍生物是等效的免疫原。
