Mohamed A J, Sathiyaraj D, Muthu R
Department of Genetics, Post Graduate Institute of Basic Medical Sciences, Madras, India.
Jpn J Med Sci Biol. 1997 Feb;50(1):1-7. doi: 10.7883/yoken1952.50.1.
We have used the cell wall-associated proteins of Mycobacterium tuberculosis (SIHV strain) as antigens in the serodiagnosis of pulmonary tuberculosis. Sera from 19 relapsed and 46 newly diagnosed cases of pulmonary tuberculosis and 21 healthy individuals were tested against the cell wall-associated proteins of M. tuberculosis (SIHV) by an ELISA technique. The results showed 92% and 100% positive titers in new and relapsed cases of tuberculosis, respectively. Control sera analyzed exhibited a negativity of 90%. Cell wall-associated proteins of M. tuberculosis were found to be useful in serodiagnosis of pulmonary tuberculosis in clear distinction from healthy subjects.
我们已将结核分枝杆菌（SIHV 菌株）的细胞壁相关蛋白用作肺结核血清诊断中的抗原。采用酶联免疫吸附测定（ELISA）技术，对 19 例复发肺结核病例、46 例新诊断肺结核病例的血清以及 21 名健康个体的血清进行了检测，以检测其对结核分枝杆菌（SIHV）细胞壁相关蛋白的反应。结果显示，新诊断肺结核病例和复发肺结核病例的阳性滴度分别为 92%和 100%。所分析的对照血清阴性率为 90%。结果表明，结核分枝杆菌的细胞壁相关蛋白在肺结核血清诊断中具有重要作用，与健康受试者有明显区别。
