Jagetia G C, Aruna R
Department of Radiobiology, Kasturba Medical College, Manipal, India.
Mutat Res. 1997 Sep 18;393(1-2):157-63. doi: 10.1016/s1383-5718(97)00101-0.
The induction of micronuclei was studied in mouse bone marrow treated or not with 5, 10 and 20 mg/kg b.wt. of abana (a herbal preparation) before exposure to 0-3 Gy of gamma-radiation. Whole-body irradiation of mice resulted in a dose-dependent increase in the frequency of micronuclei. Treatment of mice with various doses of abana before exposure to different doses of gamma-rays resulted in a significant reduction of the micronucleus frequency at all exposure doses. The highest decline in the frequencies of micronuclei was observed after administration of 20 mg/kg abana, where the frequency of micronuclei was approximately 4-fold less than that of the concurrent control. The PCE/NCE ratio was significantly higher in the drug-treated group compared to DDW + irradiated control and it was almost restored to normal level after administration of 20 mg/kg abana. Our results demonstrate that abana protects mice against radiation-induced micronucleus formation and radiation-induced decline in cell proliferation.
在小鼠暴露于0 - 3 Gy的γ射线之前，研究了用5、10和20 mg/kg体重的阿巴纳（一种草药制剂）处理或未处理的小鼠骨髓中微核的诱导情况。对小鼠进行全身照射导致微核频率呈剂量依赖性增加。在暴露于不同剂量的γ射线之前，用不同剂量的阿巴纳处理小鼠，在所有暴露剂量下微核频率均显著降低。在给予20 mg/kg阿巴纳后，观察到微核频率下降幅度最大，此时微核频率比同期对照组低约4倍。与蒸馏水 + 照射对照组相比，药物处理组的嗜多染红细胞/正常红细胞比值显著更高，在给予20 mg/kg阿巴纳后该比值几乎恢复到正常水平。我们的结果表明，阿巴纳可保护小鼠免受辐射诱导的微核形成以及辐射诱导的细胞增殖下降。
