Ayukawa H M, Saltzburg D, Mukherjee B B
Int J Cancer. 1979 Oct 15;24(4):498-503. doi: 10.1002/ijc.2910240419.
The effects of bromodeoxyuridine (BrdUrd) incorporation into hepatocytes of carbon tetrachloride (CCl4)-induced regenerating liver of BALB/c mice on the induction of endogenous retroviruses was examined. From the nucliec acid hybridization studies, the maximum levels of hybridization were obtained for both N- and X-tropic BALB/c endogenous retrovirus specific [3H]-cDNAs with liver RNA from animals receiving BrdUrd at 40 and 44 h post-CCl4 treatment, and killed on the fourth day following BrdUrd injection. Media from NIH-3T3 (Swiss mouse) and mink cell cultures, infected with liver homogenates from animals treated as above, gave significant levels of reverse transcriptase activity. The observations made in the present study show that BrdUrd incorporation into cell DNA can cause induction of both N- and X-tropic endogenous retroviruses in BALB/c mouse hepatocytes in vivo, and such induction is probably a transient event.
研究了溴脱氧尿苷（BrdUrd）掺入四氯化碳（CCl4）诱导的BALB/c小鼠再生肝的肝细胞中对内源性逆转录病毒诱导的影响。通过核酸杂交研究，在用CCl4处理后40和44小时接受BrdUrd并在注射BrdUrd后第四天处死的动物的肝脏RNA中，获得了N-和X-嗜性BALB/c内源性逆转录病毒特异性[3H]-cDNA的最大杂交水平。用上述处理动物的肝脏匀浆感染的NIH-3T3（瑞士小鼠）和貂细胞培养物的培养基具有显著水平的逆转录酶活性。本研究中的观察结果表明，BrdUrd掺入细胞DNA可在体内诱导BALB/c小鼠肝细胞中的N-和X-嗜性内源性逆转录病毒，并且这种诱导可能是一个短暂的事件。
