Vincent R N, Mukherjee B B, Mobry P M, Bartok K
J Gen Virol. 1976 Dec;33(3):411-9. doi: 10.1099/0022-1317-33-3-411.
In the present study the extent of endogenous, type-C virus genome transcription in normal and regenerating mouse liver was analysed by using the technique of nucleic acid hybridization. The RNA preparations from regenerating liver tissues collected at various intervals following partial hepatectomy, and from normal liver samples of BALB/c mice, were hybridized to 3H-DNA complementary to 60 to 70S RNA of an endogenous, N-tropic virus, released spontaneously from BALB/c mouse cells in culture. Although partial transcription of the endogenous virus genome can be clearly detected in normal liver, a significant increase in the level of virus-specific RNA synthesis in the regenerating liver, in comparison to normal liver, is apparent, following partial hepatectomy. This increase in virus-specific RNA synthesis attains its highest level just before the level of DNA synthesis in the regenerating liver reaches its maximum. These observations may indicate a qualitative or quantitative change in the endogenous type-C virus genome transcription pattern in hepatocytes, in response to partial hepatectomy and suggest that this change in the transcription pattern and the initiation of cell proliferation, in regenerating livers, are probably sequential and related events.
在本研究中，运用核酸杂交技术分析了正常和再生小鼠肝脏中内源性C型病毒基因组的转录程度。将部分肝切除术后不同时间收集的再生肝组织以及BALB/c小鼠正常肝样本的RNA制剂，与3H-DNA进行杂交，该3H-DNA与一种内源性N嗜性病毒的60至70S RNA互补，此病毒是从培养的BALB/c小鼠细胞中自发释放的。虽然在正常肝脏中能清晰检测到内源性病毒基因组的部分转录，但与正常肝脏相比，部分肝切除术后再生肝脏中病毒特异性RNA合成水平显著增加。这种病毒特异性RNA合成的增加在再生肝脏中DNA合成水平达到最大值之前达到最高水平。这些观察结果可能表明，肝细胞中内源性C型病毒基因组转录模式发生了质或量的变化，以响应部分肝切除术，并提示在再生肝脏中这种转录模式的变化与细胞增殖的启动可能是相继且相关的事件。
