Cohen-Kashi M, Deutsch M, Tirosh R, Rachmani H, Weinreb A
Jeromne Schottenstein Cellscan Center for Early Detection of Cancer, Department of Physics, Bar-Ilan University, Ramat-Gan, Israel.
Spectrochim Acta A Mol Biomol Spectrosc. 1997 Sep;53A(10):1655-61. doi: 10.1016/s1386-1425(97)00102-9.
The application of carboxyfluorescein (CF), as an impermeable fluorescent probe for lymphocyte stimulation with phytohaemagglutinin (PHA), is investigated by following a decrease in the degree of fluorescence polarization. Since CF does not enter the mitochondria, the present results indicate that the measured effect of stimulation occurs in the cytoplasm. The results also reveal that the fluorescence yield of intracellular CF is smaller than that of extracellular CF. Moreover, the degree of fluorescence polarization of intracellular CF is inversely related to its concentration. Following cell disruption, fluorescence intensity increases and polarization decreases. These effects might indicate a weak or reversible association of intracellular CF with cytoplasmic proteins.
研究了羧基荧光素（CF）作为一种不透膜的荧光探针，用于检测植物血凝素（PHA）刺激淋巴细胞时荧光偏振度的降低情况。由于CF不进入线粒体，目前的结果表明，所测得的刺激效应发生在细胞质中。结果还显示，细胞内CF的荧光产率低于细胞外CF。此外，细胞内CF的荧光偏振度与其浓度呈负相关。细胞破裂后，荧光强度增加而偏振度降低。这些效应可能表明细胞内CF与细胞质蛋白存在弱的或可逆的结合。
