Hashimoto Y, Shinozaki N
Department of Internal Medicine, Yokohama Seamen's Hospital, Kanagawa, Japan.
J Histochem Cytochem. 1988 Jun;36(6):609-13. doi: 10.1177/36.6.3367046.
We have developed an improved technique of fluorescence polarization for measurement of the cytoplasmic viscosity of human peripheral lymphocytes. When measured using this improved method, the mean value of fluorescence polarization in lymphocytes from 20 healthy donors was 0.190 +/- 0.005. The mean value of fluorescence polarization as percent of control after stimulation of lymphocytes with phytohemagglutinin (PHA) was 83.6 +/- 3.8%. We discuss here optimal conditions for PHA stimulation of lymphocytes.
我们已经开发出一种改进的荧光偏振技术，用于测量人外周血淋巴细胞的细胞质粘度。当使用这种改进方法进行测量时，来自20名健康供体的淋巴细胞中荧光偏振的平均值为0.190±0.005。用植物血凝素（PHA）刺激淋巴细胞后，作为对照百分比的荧光偏振平均值为83.6±3.8%。我们在此讨论PHA刺激淋巴细胞的最佳条件。
