Tenzaki T, Ishiguro T
Nihon Gan Chiryo Gakkai Shi. 1989 Apr 20;24(4):817-25.
Percoll gradient (density: 1.081 g/cm3) centrifugation of peripheral venous blood yielded two layers of mononuclear cells. Preliminary study using lower lymphocyte layer revealed that there were significant differences in lymphocyte fluorescence polarization changes after PHA stimulation between malignant conditions and benign or healthy conditions, while such differences were not apparent by use of upper lymphocyte layer. According to these preliminary results, we studied fluorescence polarization changes after PHA stimulation in lower lymphocyte layer obtained from 242 individuals, including 49 apparently healthy women, 93 patients with benign gynecological tumor, 17 patients with severe dysplasia of the uterine cervix and 83 patients with malignant gynecological tumor. Stimulation index (R) was defined as the ratio between the degree of polarization before PHA stimulation (P CONTROL) and the degree of polarization after PHA stimulation (PPHA): R = PPHA/PCONTROL. R values of less than unity (R less than 1) indicate stimulation with PHA, and R values of greater than unity (R greater than or equal to 1), non-stimulation with PHA, respectively. R values of less than unity (R less than 1) was found in 118 of 142 (83.1%) healthy women or patients with benign tumor, 5 of 17 (29.4%) patients with cervical dysplasia, and 13 of 83 (15.7%) patients with malignant tumor, respectively. Preoperative R values of greater than unity decreased to R values of less than unity in 17 of 20 (85.0%) patients who received complete resection of the malignant lesion, while decline of R values was noted only in 8 of 22 (36.4%) patients who received incomplete resection of the lesion and/or chemotherapy or radiation. Present findings indicate that fluorescence polarization changes after PHA stimulation in lymphocytes isolated by Percoll gradient facilitate feasible detection of various malignancies.(ABSTRACT TRUNCATED AT 250 WORDS)
采用Percoll梯度（密度：1.081 g/cm³）离心外周静脉血，得到两层单核细胞。对下层淋巴细胞层进行的初步研究显示，恶性疾病与良性或健康状况相比，PHA刺激后淋巴细胞荧光偏振变化存在显著差异，而使用上层淋巴细胞层时这种差异并不明显。根据这些初步结果，我们研究了从242名个体获取的下层淋巴细胞层在PHA刺激后的荧光偏振变化，这些个体包括49名表面健康的女性、93名良性妇科肿瘤患者、17名子宫颈重度发育异常患者和83名恶性妇科肿瘤患者。刺激指数（R）定义为PHA刺激前的偏振度（P CONTROL）与PHA刺激后的偏振度（PPHA）之比：R = PPHA/PCONTROL。R值小于1表示PHA刺激，R值大于或等于1表示未受PHA刺激。在142名健康女性或良性肿瘤患者中的118名（83.1%）、17名宫颈发育异常患者中的5名（29.4%）以及83名恶性肿瘤患者中的13名（15.7%）中发现R值小于1。20名接受恶性病变完全切除的患者中有17名（85.0%）术前大于1的R值降至小于1，而在22名接受病变不完全切除和/或化疗或放疗的患者中，只有8名（36.4%）出现R值下降。目前的研究结果表明，通过Percoll梯度分离的淋巴细胞在PHA刺激后的荧光偏振变化有助于对各种恶性肿瘤进行可行的检测。（摘要截短至250字）
