艾哈迈德前房分流器及自制前房分流器的体外血流特性

In vitro flow characteristics of the Ahmed and self-constructed anterior chamber shunts.

作者信息

Strubbe D T, Gelatt K N, MacKay E O

机构信息

Gwathmey-Adams Laboratory for Visual Science, College of Veterinary Medicine, University of Florida, Gainesville 32610-0126, USA.

出版信息

Am J Vet Res. 1997 Nov;58(11):1332-7.

PMID:9361902

Abstract

OBJECTIVE

To compare in vitro opening pressures (OP) and closing pressures (CP) of the Ahmed VS-1 and VS-2 glaucoma valves with those of several self-constructed valve 'prototypes,' and to assess their ability to maintain perfusion pressures between 6 and 21 mm of Hg.

SAMPLE POPULATION

Ahmed VS-1 (n = 6), 2 groups of Ahmed VS-2 (group 1: n = 12; group 2: n = 14), and self-constructed valves with linear incisions in the long axis of the tube wall (n = 6) or X-shaped incisions in the tube walls (n = 2).

PROCEDURE

Valves were perfused with deionized water, lactated Ringer's solution (LRS), Dulbecco's modified Eagle's medium (DMEM), DMEM plus 50% equine serum (ES), and 100% ES. Flow rates of 2.85, 4.2, 6.0, 9.0, and 12.0 microliter/min were used for each perfusate. Valves were tested 3 times for reproducibility, and OP/CP were compared for each system.

RESULTS

OP/CP of the VS-1, VS-2 (group 1), VS-2 (group 2), and linear 1.0-cm incisional valves with thick tubing consistently increased with increasing perfusion rate. Linear 0.5-cm (thick tubing) and 1.0-cm (thin tubing) incisional valves had increasing OP/CP with increasing perfusion rate in all but a few instances. Mean OP/CP decreased with increasing perfusate osmolarity for all perfusates except LRS, using the VS-1 and V-2 (group 2) valves. Mean OP/CP were consistently lower for VS-1 than VS-2 (group 1) valves at any given flow rate and for any given perfusate. Mean OP/CP were consistently lower for VS-2 (group 1) than VS-2 (group 2) valves at any given flow rate and for any given perfusate. The linear 0.5-cm incisional valves with thick and thin tubing induced the highest mean OP/CP, maximizing at > 30 mm of Hg.

CONCLUSIONS

Only the VS-2 (group 2) valves consistently had mean OP/CP between 6 and 21 mm of Hg for all perfusates and at all flow rates.

CLINICAL RELEVANCE

Anterior chamber shunts, although imperfect, appear to offer a physiologically sound alternative for glaucoma management.

摘要

目的

比较艾哈迈德VS - 1和VS - 2青光眼引流阀与几种自行构建的瓣膜“原型”的体外开启压力（OP）和关闭压力（CP），并评估它们维持6至21毫米汞柱灌注压力的能力。

样本群体

艾哈迈德VS - 1（n = 6），两组艾哈迈德VS - 2（第1组：n = 12；第2组：n = 14），以及在管壁长轴上有线性切口的自行构建瓣膜（n = 6）或在管壁上有X形切口的自行构建瓣膜（n = 2）。

步骤

用去离子水、乳酸林格氏液（LRS）、杜尔贝科改良伊格尔培养基（DMEM）、含50%马血清（ES）的DMEM以及100% ES对瓣膜进行灌注。每种灌注液使用2.85、4.2、6.0、9.0和12.0微升/分钟的流速。对瓣膜进行3次测试以评估可重复性，并比较每个系统的OP/CP。

结果

VS - 1、VS - 2（第1组）、VS - 2（第2组）以及带有厚壁管的1.0厘米线性切口瓣膜的OP/CP随灌注速率增加而持续升高。带有厚壁管的0.5厘米线性切口瓣膜和带有薄壁管的1.0厘米线性切口瓣膜在除少数情况外，其OP/CP也随灌注速率增加。除LRS外，使用VS - 1和VS - 2（第2组）瓣膜时，所有灌注液的平均OP/CP随灌注液渗透压升高而降低。在任何给定流速和任何给定灌注液条件下，VS - 1瓣膜的平均OP/CP始终低于VS - 2（第1组）瓣膜。在任何给定流速和任何给定灌注液条件下，VS - 2（第1组）瓣膜的平均OP/CP始终低于VS - 2（第2组）瓣膜。带有厚壁管和薄壁管的0.5厘米线性切口瓣膜诱导出最高的平均OP/CP，在> 30毫米汞柱时达到最大值。