Cluett E B, Kuismanen E, Machamer C E
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2105, USA.
Mol Biol Cell. 1997 Nov;8(11):2233-40. doi: 10.1091/mbc.8.11.2233.
To investigate the distribution of lipids through the Golgi complex, we analyzed the envelopes of several viruses that assemble in different subcompartments of the Golgi, as well as subcellular fractions. Our results indicate that each Golgi subcompartment has a distinct phospholipid composition due mainly to differences in the relative amounts of semilysobisphosphatidic acid (SLBPA), sphingomyelin, phosphatidylserine, and phosphatidylinositol. Interestingly, SLBPA is enriched in the adjacent Golgi networks compared with the Golgi stack, and this enrichment varies with cell type. The heterogeneous distribution of SLBPA through the Golgi complex suggests it may play an important role in the structure and/or function of this organelle.
为了研究脂质在高尔基体中的分布，我们分析了几种在高尔基体不同亚区组装的病毒包膜以及亚细胞组分。我们的结果表明，每个高尔基体亚区都有独特的磷脂组成，这主要是由于半溶血双磷脂酸（SLBPA）、鞘磷脂、磷脂酰丝氨酸和磷脂酰肌醇相对含量的差异。有趣的是，与高尔基体堆叠相比，SLBPA在相邻的高尔基体网络中更为富集，且这种富集因细胞类型而异。SLBPA在高尔基体中的异质分布表明它可能在该细胞器的结构和/或功能中发挥重要作用。
