Kivelä J, Parkkila S, Metteri J, Parkkila A K, Toivanen A, Rajaniemi H
Parolannummi Garrison Hospital, Finnish Defence Forces, Hattula, Finland.
Acta Physiol Scand. 1997 Oct;161(2):221-5. doi: 10.1046/j.1365-201X.1997.00217.x.
Two successive saliva samples were collected under strictly standardized conditions from 209 healthy, selected male soldiers prior to and after breakfast in the morning and were assayed for carbonic anhydrase (CA) VI concentrations using a specific time-resolved immunofluorometric assay. Salivary secretion rates, pH and buffer capacity pH values, and amylase activity levels were also determined. CA VI concentrations correlated positively to salivary secretion rates and amylase activity levels. By contrast, no significant correlation was seen between CA VI concentrations and pH or buffer capacity pH values, nor between amylase activity levels and salivary secretion rates, pH or buffer capacity pH values. The smokers had unaltered salivary secretion rates, CA VI and amylase activity levels, but lower salivary pH and buffer capacity pH values than the non-smokers. Present results suggest that salivary CA VI is not directly involved in the regulation of pH in saliva.
在严格标准化的条件下，于早餐前后从209名经过挑选的健康男性士兵中采集两份连续的唾液样本，采用特定的时间分辨免疫荧光分析法测定碳酸酐酶（CA）VI浓度。还测定了唾液分泌率、pH值和缓冲容量pH值以及淀粉酶活性水平。CA VI浓度与唾液分泌率和淀粉酶活性水平呈正相关。相比之下，CA VI浓度与pH值或缓冲容量pH值之间，以及淀粉酶活性水平与唾液分泌率、pH值或缓冲容量pH值之间均未观察到显著相关性。吸烟者的唾液分泌率、CA VI和淀粉酶活性水平未改变，但唾液pH值和缓冲容量pH值低于不吸烟者。目前的结果表明，唾液CA VI不直接参与唾液pH值的调节。
