Kivelä Jyrki, Laine Merja, Parkkila Seppo, Rajaniemi Hannu
Department of Anatomy and Cell Biology, University of Oulu, FIN-90014 Oulu, Finland.
Arch Oral Biol. 2003 Aug;48(8):547-51. doi: 10.1016/s0003-9969(03)00096-7.
Previous studies have shown that pregnancy may have unfavourable effects on oral health. The pH and buffer capacity (BC) of paraffin-stimulated saliva, for example, have been found to decrease towards late pregnancy. Salivary carbonic anhydrase VI (CA VI) probably protects the teeth by accelerating the neutralization of hydrogen ions in the enamel pellicle on dental surfaces. Since estrogens and androgens are known to regulate CA expression in some tissues, we studied here whether salivary CA VI concentration shows pregnancy-related changes.
Paraffin-stimulated salivary samples were collected from nine pregnant women 1 month before delivery and about 2 months afterwards and assayed for salivary CA VI concentration, BC and flow rate. The enzyme concentration was determined using a specific time-resolved immunofluorometric assay. The control group consisted of 17 healthy non-pregnant women.
The results indicated that salivary CA VI levels varied markedly among individuals, but no significant differences in mean concentrations were seen between the samples collected during late pregnancy and postpartum. BC values were lower during pregnancy, however.
Our findings suggest that CA VI secretion is not significantly affected by the hormonal alterations associated with pregnancy, and confirm the earlier reports that CA VI is not involved in the regulation of actual salivary BC.
先前的研究表明，怀孕可能对口腔健康产生不利影响。例如，已发现石蜡刺激唾液的pH值和缓冲能力（BC）在怀孕后期会降低。唾液碳酸酐酶VI（CA VI）可能通过加速牙表面釉质 pellicle 中氢离子的中和来保护牙齿。由于已知雌激素和雄激素可调节某些组织中的CA表达，我们在此研究唾液CA VI浓度是否显示与怀孕相关的变化。
从9名孕妇在分娩前1个月和产后约2个月收集石蜡刺激的唾液样本，并检测唾液CA VI浓度、BC和流速。使用特定的时间分辨免疫荧光测定法测定酶浓度。对照组由17名健康未怀孕女性组成。
结果表明，唾液CA VI水平在个体间差异显著，但怀孕后期和产后收集的样本之间平均浓度无显著差异。然而，怀孕期间BC值较低。
我们的研究结果表明，CA VI分泌不受与怀孕相关的激素变化的显著影响，并证实了早期报告，即CA VI不参与实际唾液BC的调节。
