Halim A B, el-Ahmady O, Hassab-Allah S, Abdel-Galil F, Hafez Y, Darwish A
Tumor Marker Oncology Research Center, Al-Azhar University, Cairo, Egypt.
Ann Clin Biochem. 1997 Nov;34 ( Pt 6):656-63. doi: 10.1177/000456329703400610.
Recent studies demonstrated the role of antioxidants in preventing organ damage caused by free radicals. The present study was conducted to find out the modulatory effect of some antioxidants on lipid patterns in experimentally-induced liver damage. Rats chronically intoxicated with carbon tetrachloride (CCl4) were used as a model of liver injury terminating with fibrosis or cirrhosis. One hundred and sixty six albino rats were classified into five groups: one served as a control group; the second was subjected to oral administration of CCl4 (200 microL/100 g body weight) twice a week; the other three groups, in addition to CCl4, received oral doses of silymarin (30 mg/kg), vitamin E (200 IU/kg) and vitamin C (50 mg/kg) respectively. At the end of the experiment, the animals were killed, blood was collected and liver was taken for histopathological examination. Liver function tests, disturbed by CCl4 were significantly modulated by antioxidants, and histopathological examination showed that antioxidants ameliorated the necrotic and fibrotic changes caused by CCl4. Treatment with antioxidants was also shown to modulate the toxic effect of CCl4 on the lipid profile and malondialdehyde content. Administration of antioxidants could play an important role in prophylaxis against lipid peroxidation and consequently liver fibrosis caused by free radicals.
近期研究表明抗氧化剂在预防自由基引起的器官损伤中发挥着作用。本研究旨在探究某些抗氧化剂对实验性诱导肝损伤中脂质模式的调节作用。将长期用四氯化碳（CCl4）中毒的大鼠作为肝损伤模型，最终发展为纤维化或肝硬化。166只白化大鼠分为五组：一组作为对照组；第二组每周两次口服CCl4（200微升/100克体重）；另外三组除CCl4外，分别口服水飞蓟宾（30毫克/千克）、维生素E（200国际单位/千克）和维生素C（50毫克/千克）。实验结束时，处死动物，采集血液并取肝脏进行组织病理学检查。受CCl4干扰的肝功能测试被抗氧化剂显著调节，组织病理学检查显示抗氧化剂改善了CCl4引起的坏死和纤维化变化。抗氧化剂治疗还显示可调节CCl4对脂质谱和丙二醛含量的毒性作用。给予抗氧化剂在预防脂质过氧化以及由此引起的自由基诱导的肝纤维化方面可能发挥重要作用。
