Chang C W, Liao Y K, Su V, Farh L, Shiuan D
Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.
Biochem Mol Biol Int. 1998 Aug;45(5):979-87. doi: 10.1002/iub.7510450515.
The nucleotide sequences of the S-RNA of Akabane viruses JaGAr-39, OBE-1, Iriki and the newly isolated PT-17 strains and the Aino virus were determined and compared. The results reveal that the S-RNAs of the four Akabane strains share 96.9% homology in nucleotide sequences. Only one amino acid difference out of the 233 amino acids of the nucleocapsid protein (N) and three amino acid differences in the 91 amino acids of the nonstructural protein (NSs) were found among the Akabane viruses. Amino acid sequences of N and NSs proteins of the Aino virus have approximately 80% identity as compared with the Akabane viruses. The results also demonstrate that the four Akabane viruses and the Aino virus can be clearly differentiated by RFLP (restriction fragments length polymorphism) analysis using RT-PCR generated nucleocapsid protein genes and digested with HaeIII and HindIII. The phylogenetic tree based on the UPGMA (Unweighted Pair Group Method with Arithmetic Mean) analysis of the sequences of nucleocapsid protein genes and the S-DNAs revealed that the newly isolated PT-17 strain is most closely related to Iriki strain, than the JaGAr-39 or OBE-1 strains.
测定并比较了赤羽病毒JaGAr - 39、OBE - 1、入来株以及新分离的PT - 17株和艾诺病毒的S - RNA核苷酸序列。结果显示，四种赤羽病毒株的S - RNA核苷酸序列同源性为96.9%。在赤羽病毒中，核衣壳蛋白（N）的233个氨基酸中仅发现一处氨基酸差异，非结构蛋白（NSs）的91个氨基酸中发现三处氨基酸差异。与赤羽病毒相比，艾诺病毒的N蛋白和NSs蛋白氨基酸序列的同一性约为80%。结果还表明，使用RT - PCR扩增核衣壳蛋白基因并用HaeIII和HindIII酶切进行RFLP（限制性片段长度多态性）分析，可以清晰地区分四种赤羽病毒和艾诺病毒。基于核衣壳蛋白基因和S - DNA序列的UPGMA（非加权组平均法）分析构建的系统发育树显示，新分离的PT - 17株与入来株的关系比与JaGAr - 39株或OBE - 1株更为密切。
