Saeed M F, Wang H, Nunes M, Vasconcelos P F, Weaver S C, Shope R E, Watts D M, Tesh R B, Barrett A D
Department of Microbiology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USA.
J Gen Virol. 2000 Mar;81(Pt 3):743-8. doi: 10.1099/0022-1317-81-3-743.
The nucleotide sequence of the S RNA segment of the Oropouche (ORO) virus prototype strain TRVL 9760 was determined and found to be 754 nucleotides in length. In the virion-complementary orientation, the RNA contained two overlapping open reading frames of 693 and 273 nucleotides that were predicted to encode proteins of 231 and 91 amino acids, respectively. Subsequently, the nucleotide sequences of the nucleocapsid genes of 27 additional ORO virus strains, representing a 42 year interval and a wide geographical range in South America, were determined. Phylogenetic analyses revealed that all the ORO virus strains formed a monophyletic group that comprised three distinct lineages. Lineage I contained the prototype strain from Trinidad and most of the Brazilian strains, lineage II contained six Peruvian strains isolated between 1992 and 1998, and two strains from western Brazil isolated in 1991, while lineage III comprised four strains isolated in Panama during 1989.
对奥罗普切（ORO）病毒原型株TRVL 9760的S RNA片段的核苷酸序列进行了测定，发现其长度为754个核苷酸。在病毒粒子互补方向上，该RNA包含两个重叠的开放阅读框，分别为693个核苷酸和273个核苷酸，预计分别编码231个和91个氨基酸的蛋白质。随后，测定了另外27株ORO病毒核衣壳基因的核苷酸序列，这些病毒代表了南美洲42年的时间间隔和广泛的地理范围。系统发育分析表明，所有ORO病毒株形成了一个单系群，该单系群包含三个不同的谱系。谱系I包含来自特立尼达的原型株和大多数巴西株，谱系II包含1992年至1998年间分离的6株秘鲁株以及1991年在巴西西部分离的2株，而谱系III包含1989年在巴拿马分离的4株。
