Tate D J, Oliver P D, Miceli M V, Stern R, Shuster S, Newsome D A
Sensory and Electrophysiology Research Unit, Touro Infirmary, New Orleans, La.
Arch Ophthalmol. 1993 Jul;111(7):963-7. doi: 10.1001/archopht.1993.01090070083023.
Hyaluronic acid (HA) has a key role in the structure and organization of the extracellular matrix. We sought to identify the distribution of HA in human eye tissue with regard to age using a biotinylated HA-binding protein.
Fetal and adult (from donors ranging from 28 to 94 years of age) eye tissues were fixed less than 24 hours post mortem and embedded in JB-4 medium (Polysciences, Warrington, Pa). Sections of 2-microns thickness were used. Control sections were pretreated either with Streptomyces hyaluronidase or HA-binding protein inactivated by HA. The binding of the protein to HA was detected with avidinbiotin alkaline phosphatase and developed by incubation with naphthol as-mx phosphate and Texas Red Salt (Pierce, Rockford, Ill).
Specific staining for HA was observed in fetal eyes in the choroid, Bruch's membrane, sclera, retinal pigment epithelium, and developing retina from the vitreoretinal interface to the inner plexiform layer. Specific staining decreased with age in the choroid, retinal pigment epithelium, and Bruch's membrane. Hyaluronic acid-specific staining was undetectable in tissues from donors over 50 years of age.
The localization of HA in the chorioretinal complex and its disappearance after the fifth decade of life may play a role in aging and age-related retinal disorders.
透明质酸（HA）在细胞外基质的结构和组织中起关键作用。我们试图使用生物素化的HA结合蛋白来确定HA在人眼组织中的分布与年龄的关系。
胎儿和成人（供体年龄范围为28至94岁）的眼组织在死后不到24小时进行固定，并包埋在JB - 4培养基（Polysciences公司，宾夕法尼亚州沃灵顿）中。使用2微米厚的切片。对照切片用链霉菌透明质酸酶或被HA灭活的HA结合蛋白进行预处理。用抗生物素蛋白 - 生物素碱性磷酸酶检测蛋白与HA的结合，并通过与萘酚 - AS - MX磷酸盐和德克萨斯红盐（Pierce公司，伊利诺伊州罗克福德）孵育显色。
在胎儿眼中，在脉络膜、布鲁赫膜、巩膜、视网膜色素上皮以及从玻璃体视网膜界面到内网状层的发育中的视网膜中观察到HA的特异性染色。在脉络膜、视网膜色素上皮和布鲁赫膜中，特异性染色随年龄增长而减少。在50岁以上供体的组织中未检测到透明质酸特异性染色。
HA在脉络膜视网膜复合体中的定位及其在生命第五个十年后消失可能在衰老和年龄相关性视网膜疾病中起作用。
