从大麦胚中大规模分离转运核糖核酸
Large-scale isolation of tRNA from barley embryos.
作者信息
Augustyniak J, Karwowska U, Goździcka A, Labuda D, Zaradniak E
出版信息
Acta Biochim Pol. 1976;23(1):3-11.
PMID:936883
Abstract
- Large-scale isolation of tRNA from barley embryos is described, involving: phenol extraction, RNA deproteinization with the chloroform-isoamyl alcohol mixture, batch sorption on DEAE-cellulose, NaCl gradient elution of tRNA from DEAE-cellulose, and deaminoacylation of tRNA in the presence of bentonite. The procedure yielded tRNA free of protein and RNase activity. 2. The amino acid acceptor activity of the crude barley tRNA, its melting profiles and chromatographic patterns on Sephadex G-100 and BD-cellulose were similar to those of tRNA from other sources.
摘要
- 本文描述了从大麦胚中大规模分离tRNA的方法，包括：苯酚提取、用氯仿 - 异戊醇混合物进行RNA脱蛋白、在DEAE - 纤维素上进行批量吸附、从DEAE - 纤维素上用NaCl梯度洗脱tRNA以及在膨润土存在下使tRNA脱氨酰化。该方法得到了不含蛋白质和RNase活性的tRNA。2. 粗制大麦tRNA的氨基酸接受活性、其解链曲线以及在Sephadex G - 100和BD - 纤维素上的色谱图谱与其他来源的tRNA相似。
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