Senel O, Cetinkale O, Ozbay G, Ahçioğlu F, Bulan R
Department of Plastic & Reconstructive Surgery, Istanbul University, Cerrahpasa Medical Faculty, Turkey.
Ann Plast Surg. 1997 Nov;39(5):516-23. doi: 10.1097/00000637-199711000-00012.
Oxygen free radicals are produced and play an important role in ischemic injury. We therefore wished to investigate the role of free radicals on ischemic skin wound healing. For this purpose, H-shaped flaps, where the test ischemic wound is the horizontal line in the H, were created on the dorsum of the rat. To inhibit the probable hazards of free radicals, allopurinol and superoxide dismutase (SOD) were given to the animals. Most of the studied wound-healing parameters were impaired in the ischemic group. In the allopurinol-treated group, breaking strength was increased by 52% by day 7 and by 109% by day 14 (p < 0.0002 and p < 0.001), and in the SOD-treated group the increase was 69% both by days 7 and 14 of healing when compared with the ischemic control group (p < 0.003 and p < 0.002). Hydroxyproline content was increased 75% with allopurinol and 113% with SOD in the wound by day 7 (p < 0.03 and p < 0.001 respectively). SOD treatment caused a significant decrease in wound edema by day 7 of healing (p < 0.05). Histopathological evaluation revealed that in the SOD- and allopurinol-treated groups, the amount of collagen and its organization were more prominent when compared with the ischemic controls. These results show that oxygen free radicals play an important role in the failure of ischemic wound healing, and antioxidants partly improve the healing in ischemic skin wounds.
氧自由基在缺血性损伤中产生并起重要作用。因此，我们希望研究自由基在缺血性皮肤伤口愈合中的作用。为此，在大鼠背部制作了H形皮瓣，其中测试的缺血性伤口为H中的横线。为抑制自由基可能带来的危害，给动物注射了别嘌呤醇和超氧化物歧化酶（SOD）。缺血组中大多数研究的伤口愈合参数均受损。在别嘌呤醇治疗组中，到第7天时抗张强度增加了52%，到第14天时增加了109%（p<0.0002和p<0.001）；与缺血对照组相比，在SOD治疗组中，愈合第7天和第14天时抗张强度均增加了69%（p<0.003和p<0.002）。到愈合第7天时，伤口中羟脯氨酸含量用别嘌呤醇处理增加了75%，用SOD处理增加了113%（分别为p<0.03和p<0.001）。SOD治疗在愈合第7天时使伤口水肿显著减轻（p<0.05）。组织病理学评估显示，与缺血对照组相比，SOD和别嘌呤醇治疗组中胶原蛋白的量及其排列更显著。这些结果表明，氧自由基在缺血性伤口愈合失败中起重要作用，抗氧化剂可部分改善缺血性皮肤伤口的愈合。
