Carron J A, Fraser W D, Gallagher J A
Human Bone Cell Research Group, Department of Human Anatomy, University of Liverpool, UK.
Br J Cancer. 1997;76(8):1095-8. doi: 10.1038/bjc.1997.513.
Using RNA extracted from human tumour samples removed during surgery, we have analysed expression of mRNA for parathyroid hormone-related protein (PTHrP) and for the PTH/PTHrP receptor by RT-PCR in a panel of human breast and colon tumours. All but 1 of 18 breast tumour samples expressed PTHrP, whereas receptor expression was detected in 11 of these. Expression of the PTH/PTHrP receptor was found in three out of four metastatic lesions, including one sample in which no receptor was detected in the primary tumour. PTHrP expression was also detected in five colon tumours, and receptor expression detected in two of these. These results demonstrate that PTHrP and the PTHrP receptor are also co-expressed in breast tumours in vivo and provide further evidence that PTHrP may be an important autocrine/paracrine growth factor in breast cancer.
利用从手术切除的人类肿瘤样本中提取的RNA，我们通过逆转录聚合酶链反应（RT-PCR）分析了一组人类乳腺和结肠肿瘤中甲状旁腺激素相关蛋白（PTHrP）和PTH/PTHrP受体的mRNA表达。18个乳腺肿瘤样本中除1个外均表达PTHrP，而其中11个检测到受体表达。在4个转移灶中的3个发现了PTH/PTHrP受体表达，其中1个样本在原发性肿瘤中未检测到受体。在5个结肠肿瘤中也检测到PTHrP表达，其中2个检测到受体表达。这些结果表明，PTHrP和PTHrP受体在体内乳腺肿瘤中也共同表达，并进一步证明PTHrP可能是乳腺癌中一种重要的自分泌/旁分泌生长因子。
