Hansen I M
Am J Trop Med Hyg. 1976 May;25(3):422-6. doi: 10.4269/ajtmh.1976.25.422.
The present study was conducted to determine whether extracts from adult Schistosoma mansoni could be coated onto cholesterol-lecithin crystals. Of particular interest was the use of stunted schistosomes (SS) recovered from rabbits. The reactions obtained with this antigen in this slide flocculation (SF) test were compared with those employing antigens from cercariae and mature adult worms. The stunted schistosome SF test (SFSS) showed a high sensitivity and specificity while cercarial antigen showed extensive cross reaction with Trichinella spiralis, Ascaris lumbricoides, and Echinococcus granulosus antisera. Both SS and cercarial antigens reacted with syphilitic sera, but the former only with high titer sera. The antigen from mature adult worms could not be coated onto cholesterol crystals, a finding which is in agreement with previous investigations. The relative simplicity of the SFSS test and its apparent high sensitivity and specificity indicates that the test may be an ideal epidemiological tool for schistosomiasis surveys. The results obtained should encourage additional study of the procedure involved and investigation into the role of lipids in flocculation tests. In addition, the findings should stimulate immunological studies of the stunted schistosomes.
本研究旨在确定曼氏血吸虫成虫提取物能否包被在胆固醇-卵磷脂晶体上。特别令人感兴趣的是使用从兔子体内回收的发育不良的血吸虫（SS）。将该抗原在玻片絮凝试验（SF）中获得的反应与使用尾蚴和成熟成虫抗原的反应进行比较。发育不良的血吸虫玻片絮凝试验（SFSS）显示出高敏感性和特异性，而尾蚴抗原与旋毛虫、蛔虫和细粒棘球绦虫抗血清有广泛的交叉反应。SS抗原和尾蚴抗原均与梅毒血清反应，但前者仅与高滴度血清反应。成熟成虫的抗原不能包被在胆固醇晶体上，这一发现与先前的研究一致。SFSS试验相对简单，且具有明显的高敏感性和特异性，表明该试验可能是血吸虫病调查的理想流行病学工具。所得结果应促使人们对所涉及的程序进行更多研究，并对脂质在絮凝试验中的作用进行调查。此外，这些发现应激发对发育不良的血吸虫的免疫学研究。
