Johnson P W, Sieburth J M
Appl Environ Microbiol. 1976 Mar;31(3):423-32. doi: 10.1128/aem.31.3.423-432.1976.
The in situ microbiota from several aquaculture facilities with active nitrification was examined by transmission electron microscopy of thin sections for the presence of bacteria that contained intracytoplasmic membranes characteristic of the nitrifying bacteria. Colonies of bacteria with the cellular morphology of a species of Nitrosomonas were found to be present in both the culture water and in the biological filter slime of a freshwater chinook salmon (Oncorhynchus tshawytscha) culture system. bacteria in the water possessed the normal nitrosomonas type of ultrastructure, whereas similar bacteria in the slime had an aberrant morphology due to multiple invaginations of the cell wall and cyto-membranes and a significantly greater number of ribosomes. These nitrosomonas-like bacteria lysed during enrichment in commonly used media. Bacteria with the morphology of species of Nitrosomonas and Nitrosococcus were also observed in colonies in the surface slimes of marine culture systems for striped bass (Morone saxatilis) and quahaug (Mercenaria mercenaria).
通过对薄切片进行透射电子显微镜检查，研究了来自几个具有活性硝化作用的水产养殖设施的原位微生物群，以寻找含有硝化细菌特征性胞内膜的细菌。在淡水奇努克鲑鱼（Oncorhynchus tshawytscha）养殖系统的养殖水和生物滤池黏液中，均发现了具有亚硝化单胞菌属物种细胞形态的细菌菌落。水中的细菌具有正常的亚硝化单胞菌超微结构类型，而黏液中的类似细菌由于细胞壁和细胞膜的多次内陷以及核糖体数量明显增多，具有异常形态。这些类似亚硝化单胞菌的细菌在常用培养基富集过程中会裂解。在条纹鲈（Morone saxatilis）和圆蛤（Mercenaria mercenaria）海水养殖系统的表面黏液菌落中，也观察到了具有亚硝化单胞菌属和亚硝化球菌属物种形态的细菌。
