Környei J L, Vértes Z, Oszter A, Kovács S, Vértes M
Institute of Physiology, University Medical School of Pécs, Hungary.
Eur J Pharmacol. 1997 Oct 1;336(1):65-70. doi: 10.1016/s0014-2999(97)01153-9.
The effect of opioid peptides on estradiol-induced cell proliferation in adult rat uterine primary cell cultures was studied. Estradiol increased cell density by 40%. This estradiol-induced stimulation of cell proliferation was decreased to control values by [D-Met2,Pro5]enkephalinamide. The opioid-induced inhibition of uterine cell proliferation was blocked completely by the specific opiate antagonist naloxone, while naloxone did not have any effect on its own. The inhibition of cell proliferation by enkephalinamide was apparent at each stimulatory estradiol concentration examined. This opioid effect was mediated mainly by the mu opiate receptor. The observed effects occurred within the physiological nanomolar concentration range. Enkephalinamide did not have any effect on the basal proliferation rate of adult rat uterine cells. However, enkephalinamide inhibited the basal rate of cell proliferation in cell cultures prepared from 7-day-old immature rats. In summary, here we present evidence of novel physiological direct cross-talk between the opioid and estrogenic signaling systems in the regulation of normal uterine growth.
研究了阿片肽对成年大鼠子宫原代细胞培养物中雌二醇诱导的细胞增殖的影响。雌二醇使细胞密度增加了40%。[D-蛋氨酸2，脯氨酸5]脑啡肽酰胺将这种雌二醇诱导的细胞增殖刺激降低至对照值。阿片类药物诱导的子宫细胞增殖抑制被特异性阿片拮抗剂纳洛酮完全阻断，而纳洛酮自身没有任何作用。在所检测的每个刺激雌二醇浓度下，脑啡肽酰胺对细胞增殖的抑制作用均很明显。这种阿片类药物效应主要由μ阿片受体介导。观察到的效应发生在生理纳摩尔浓度范围内。脑啡肽酰胺对成年大鼠子宫细胞的基础增殖率没有任何影响。然而，脑啡肽酰胺抑制了从7日龄未成熟大鼠制备的细胞培养物中的基础细胞增殖率。总之，我们在此提供了证据，证明在正常子宫生长的调节中，阿片类和雌激素信号系统之间存在新的生理性直接相互作用。
