Wiedmann-Al-Ahmad M, Schuessler P, Freidank H M
Abteilung Mikrobiologie und Hygiene, Institut für Medizinische Mikrobiologie und Hygiene, Klinikum der Universität Freiburg, Germany.
Clin Diagn Lab Immunol. 1997 Nov;4(6):700-4. doi: 10.1128/cdli.4.6.700-704.1997.
A recently described 54-kDa protein has been detected in six type strains and three patient isolates of Chlamydia pneumoniae by immunoblotting with sera from patients positive for antibodies to C. pneumoniae by the microimmunofluorescence test. This protein was not found in either C. trachomatis E or C. psittaci Z 432 as an antigen, confirming its species specificity. The 54-kDa protein was isolated by continuous-elution electrophoresis and immunoglobulin G monoclonal antibodies (MAbs) against the isolated antigen were produced. MAb 8B11E6 reacted only with the 54-kDa band of C. pneumoniae and not with C. trachomatis E or C. psittaci in a Western immunoblot assay. This antibody was purified and tested for neutralizing activity together with three additional anti-p54-active MAbs (8B11E6, 8B11B4, and 10F1C1). In Buffalo green monkey cells, all of the MAbs significantly reduced the infectivity of C. pneumoniae elementary bodies, whereas no neutralizing activity could be observed with C. trachomatis E or C. psittaci Z 432. These results not only confirm the species specificity of the 54-kDa protein but also indicate that this protein might play an important role in the pathogenesis of C. pneumoniae infection. Furthermore, the results suggest a possible protective role of anti-p54 antibodies in an adaptive immune response.
通过用微量免疫荧光试验检测肺炎衣原体抗体呈阳性的患者血清进行免疫印迹分析，在6株肺炎衣原体标准菌株和3株患者分离株中检测到一种最近描述的54 kDa蛋白。沙眼衣原体E株或鹦鹉热衣原体Z432株中均未发现该蛋白作为抗原，证实了其种属特异性。通过连续洗脱电泳分离出54 kDa蛋白，并制备了针对分离抗原的免疫球蛋白G单克隆抗体（MAb）。在Western免疫印迹分析中，单克隆抗体8B11E6仅与肺炎衣原体的54 kDa条带反应，而不与沙眼衣原体E株或鹦鹉热衣原体反应。纯化该抗体，并与另外三种抗p54活性单克隆抗体（8B11E6、8B11B4和10F1C1）一起测试中和活性。在布法罗绿猴细胞中，所有单克隆抗体均显著降低了肺炎衣原体原体的感染性，而沙眼衣原体E株或鹦鹉热衣原体Z432株未观察到中和活性。这些结果不仅证实了54 kDa蛋白的种属特异性，还表明该蛋白可能在肺炎衣原体感染的发病机制中起重要作用。此外，结果提示抗p54抗体在适应性免疫反应中可能具有保护作用。
