Freidank H M, Clad A, Herr A S, Wiedmann-Al-Ahmad M, Jung B
Institut für Medizinische Mikrobiologie und Hygiene, Freiburg, Germany.
Eur J Clin Microbiol Infect Dis. 1995 Dec;14(12):1063-9. doi: 10.1007/BF01590940.
A total of 446 sera from 245 patients with primary or secondary infertility, all of whom were examined laparoscopically, 117 patients with Chlamydia trachomatis-positive cervical swabs, and 84 control persons (50 obstetric patients and 34 female blood donors) were tested for antibodies to Chlamydia trachomatis and to Chlamydia pneumoniae with the microimmunofluorescence (MIF) test. MIF test antibody rates were highest in patients with complete tubal occlusion (73%) and in patients with proven Chlamydia trachomatis infection (74%), whereas only 9 to 10% of the control group showed Chlamydia trachomatis antibodies. Reaction to the 60 kDa antigen of Chlamydia trachomatis, a heat-shock protein (hsp) analogue, has been suggested as a possible marker for the development of chronic sequelae after Chlamydia trachomatis infection. Immunoblot analysis of 222 sera (169 infertility patients, 20 antigen-positive patients, and 33 mothers) showed a significantly higher anti-hsp antibody rate in patients with complete tubal occlusion than in infertility patients with normal fallopian tubes (76% vs. 19%, p < 0.001). The presence of antibodies not only to Chlamydia trachomatis but also to Chlamydia pneumoniae in the MIF test was associated with a significantly higher rate of anti-hsp antibodies and with complete tubal occlusion. This association did not appear to be due to cross-reactivity between Chlamydia pneumoniae and Chlamydia trachomatis antibodies in the MIF test.
对245例原发性或继发性不孕症患者（均接受了腹腔镜检查）的446份血清、117例沙眼衣原体宫颈拭子阳性患者的血清以及84名对照者（50例产科患者和34名女性献血者）进行了检测，采用微量免疫荧光（MIF）试验检测其抗沙眼衣原体和抗肺炎衣原体抗体。MIF试验抗体阳性率在输卵管完全阻塞患者中最高（73%），在已证实沙眼衣原体感染的患者中也最高（74%），而对照组中只有9%至10%的人显示有沙眼衣原体抗体。沙眼衣原体60 kDa抗原是一种热休克蛋白（hsp）类似物，对该抗原的反应被认为可能是沙眼衣原体感染后慢性后遗症发生的一个标志物。对222份血清（169例不孕症患者、20例抗原阳性患者和33名母亲）进行免疫印迹分析显示，输卵管完全阻塞患者的抗hsp抗体阳性率显著高于输卵管正常的不孕症患者（76%对19%，p<0.001）。MIF试验中不仅抗沙眼衣原体抗体而且抗肺炎衣原体抗体的存在与抗hsp抗体阳性率显著升高以及输卵管完全阻塞有关。这种关联似乎不是由于MIF试验中肺炎衣原体和沙眼衣原体抗体之间的交叉反应所致。
