Sloane J A, Pietropaolo M F, Rosene D L, Moss M B, Peters A, Kemper T, Abraham C R
Department of Pathology, Boston University School of Medicine, MA 02118, USA.
Acta Neuropathol. 1997 Nov;94(5):471-8. doi: 10.1007/s004010050735.
To assess whether amyloid plaque accumulation in the monkey brain can account for age-related cognitive impairment that begins at about 20 years of age, we measured plaque content in the brains of 14 rhesus monkeys aged 5-30 years. We used immunohistochemistry employing the monoclonal antibody 6E10, which is specific to amino acids 1-17 of the amyloid beta peptide to identify amyloid plaques in serial coronal sections of the forebrain. Amyloid plaques accumulate with age, starting at 25 years of age and escalating after 30 years. Until the age of 30, plaques are only found in a few monkeys and are relatively sparse. Results from our group and others show that plaque content and the proportion of individuals afflicted with amyloid plaques increase with age. Although both cognitive dysfunction and plaque content increase with age, amyloid plaque content does not correlate with the cognitive dysfunction observed in elderly monkeys since even in very old subjects some cognitively impaired animals have few amyloid plaques and others with abundant plaques show only minor cognitive impairments. In summary, amyloid plaques appear to accumulate significantly only in monkeys over 25 years of age but do not appear to be a causal factor in age-related cognitive decline of the normal aging rhesus monkey.
为了评估猴脑中淀粉样斑块的积累是否能解释始于约20岁的与年龄相关的认知障碍，我们测量了14只5至30岁恒河猴大脑中的斑块含量。我们使用免疫组织化学方法，采用对淀粉样β肽1 - 17氨基酸具有特异性的单克隆抗体6E10，来识别前脑连续冠状切片中的淀粉样斑块。淀粉样斑块随年龄积累，从25岁开始，30岁后加剧。在30岁之前，仅在少数猴子中发现斑块，且相对稀少。我们团队和其他团队的结果表明，斑块含量以及患有淀粉样斑块的个体比例随年龄增加。尽管认知功能障碍和斑块含量都随年龄增加，但淀粉样斑块含量与老年猴子中观察到的认知功能障碍并无关联，因为即使在非常年老的个体中，一些认知受损的动物淀粉样斑块很少，而其他斑块丰富的动物仅表现出轻微的认知障碍。总之，淀粉样斑块似乎仅在25岁以上的猴子中显著积累，但似乎不是正常衰老的恒河猴与年龄相关的认知衰退的因果因素。
