[Electroacupuncture at "huatuo jiaji point" inhibits the expression of Fos protein in rat spinal cord induced by traumatic pain].

SD rats were used to study the change of Fos-like immunoreactivity (FLI) in the lumbar spinal cord by traumatic pain following hind limb amputation and the effect of electro-acupuncture (EA) at "Huatuo Jiaji point" on it. The results showed (1) FLI cells induced by amputation were found in all of the I-X laminae of the ipsilateral spinal cord, but most of the labeled cells were located in the laminae I-II and V-VI; (2) EA at "Huatuo Jiaji point" suppressed the Fos expression following amputation, which was indicated by decrease of the number of FLI cells in the spinal cord. It suggests that, the traumatic pain impulse following amputation could activate pain sensitive neurons (PSN) in spinal cord. The impulse of electro-stimulation could suppress activities of these PSN, producing the effect of analgesia.