Carlson L A, Walldius G
Eur J Clin Invest. 1976 Jun 21;6(3):195-211. doi: 10.1111/j.1365-2362.1976.tb00512.x.
The fatty acid and glucose incorporation into glycerides and glycerol release from adipose tissue were determined in a middle-aged population of 109 men and 41 women. 43 men and 19 women were normolipidaemic. The same analysis was also carried out in 13 male and 9 female normolipidaemic students. Needle biopsy specimens of adipose tissue were incubated in vitro in an albumin medium containing 3H-fatty acids and 14C-glucose. After two hours of incubation values for fatty acid and glucose incorporation were calculated from the incorporation of 3H-activity into the fatty acids and 14C-activity into the glycerol moiety of extracted glycerides. The mean values for fatty acid incorporation were lower in all types of hypertriglyceridaemic subjects (II B, III, IV and V) than in the normolipidaemic control subjects. In the male hypertriglyceridaemic population 36% had values for fatty acid incorporation below the 5th percentile of the normolipidaemic group and 14% had values below the lowest normal value. The rate of fatty acid incorporation was negatively correlated with the serum triglyceride concentration. This correlation remained unchanged when partial correlation was performed when the influence of body weight was eliminated. Fatty acid and glucose incorporation correlated positively. Incorporation of glucose behaved in the same way as described above for incorporation of fatty acids. Glycerol and fatty acid release was the same in the normo- and hypertriglyceridaemic groups. It is likely that the removal of plasma triglycerides from blood requires hydrolysis of triglycerides to fatty acids and the subsequent removal of the fatty acids. The hypothesis has been formulated that when the former process is normal, a defect of fatty acid removal (a low rate of fatty acid incorporation into glycerides) may be responsible for an impaired removal of plasma triglyceride-fatty acids. A low rate of fatty acid incorporation may contribute to the development of hypertriglyceridaemia, according to this hypothesis.
在109名男性和41名女性的中年人群中测定了脂肪酸和葡萄糖掺入甘油酯以及脂肪组织中甘油的释放情况。43名男性和19名女性血脂正常。还对13名男性和9名女性血脂正常的学生进行了同样的分析。将脂肪组织的针吸活检标本在含有3H-脂肪酸和14C-葡萄糖的白蛋白培养基中进行体外培养。培养两小时后，根据3H活性掺入脂肪酸以及14C活性掺入提取甘油酯的甘油部分的情况，计算脂肪酸和葡萄糖的掺入值。所有类型的高甘油三酯血症患者（II B型、III型、IV型和V型）的脂肪酸掺入平均值均低于血脂正常的对照受试者。在男性高甘油三酯血症人群中，36%的脂肪酸掺入值低于血脂正常组的第5百分位数，14%的脂肪酸掺入值低于最低正常值。脂肪酸掺入率与血清甘油三酯浓度呈负相关。在消除体重影响进行偏相关分析时，这种相关性保持不变。脂肪酸和葡萄糖的掺入呈正相关。葡萄糖的掺入情况与上述脂肪酸掺入情况相同。甘油和脂肪酸的释放在血脂正常组和高甘油三酯血症组中相同。从血液中清除血浆甘油三酯可能需要将甘油三酯水解为脂肪酸，随后清除脂肪酸。有人提出这样的假说：当前一个过程正常时，脂肪酸清除缺陷（脂肪酸掺入甘油酯的速率较低）可能导致血浆甘油三酯-脂肪酸清除受损。根据这一假说，脂肪酸掺入率较低可能促成高甘油三酯血症的发生。
