Walldius G, Rubba P
Scand J Clin Lab Invest. 1976 Jul;36(4):357-69. doi: 10.3109/00365517609055271.
A method for determination of fatty acid (FIAT) and glucose (GLIAT) incorporation into adipose tissue in vitro in needle biopsy specimens of human fat has been developed. 20-150 mg of subcutaneous fat is incubated in an albumin buffer containing a physiological spectrum and concentration of fatty acids and glucose. Release of glycerol and fatty acids to the incubation medium and incorporation of labelled palmitic acid and labelled glucose into extracted adipose tissue lipids are determined simultaneously. The labelled fatty acids are found in the fatty acid part and the labelled glucose only in the glycerol part of extracted diglycerides and triglycerides. These glycerides are completely recovered and indicated FIAT and GLIAT values. Methodological errors for all vaiables are about 10%. All processes increase linearly with tissue weight and incubation time. FIAT and GLIAT increase linearly with increasing concentration of a physiological spectrum of fatty acids (=constant fractional incorporation). The method is simple, and several analyses from one subject can be performed on one day with a minimum of discomfort to the patient.
已开发出一种在人体脂肪针吸活检标本中体外测定脂肪酸（FIAT）和葡萄糖（GLIAT）掺入脂肪组织的方法。将20 - 150毫克皮下脂肪在含有生理谱和浓度的脂肪酸及葡萄糖的白蛋白缓冲液中孵育。同时测定甘油和脂肪酸释放到孵育培养基中的量以及标记的棕榈酸和标记的葡萄糖掺入提取的脂肪组织脂质中的量。在提取的甘油二酯和甘油三酯的脂肪酸部分发现标记的脂肪酸，而标记的葡萄糖仅存在于甘油部分。这些甘油酯被完全回收，并得出FIAT和GLIAT值。所有变量的方法学误差约为10%。所有过程均随组织重量和孵育时间呈线性增加。FIAT和GLIAT随生理谱脂肪酸浓度的增加呈线性增加（=恒定的分数掺入）。该方法简单，且一天内可对一名受试者进行多次分析，对患者造成的不适最小。
