Thompson Julie D, Gibson Toby J, Higgins Des G
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch Cedex, France.
Curr Protoc Bioinformatics. 2002 Aug;Chapter 2:Unit 2.3. doi: 10.1002/0471250953.bi0203s00.
The Clustal programs are widely used for carrying out automatic multiple alignment of nucleotide or amino acid sequences. The most familiar version is ClustalW, which uses a simple text menu system that is portable to more or less all computer systems. ClustalX features a graphical user interface and some powerful graphical utilities for aiding the interpretation of alignments and is the preferred version for interactive usage. Users may run Clustal remotely from several sites using the Web or the programs may be downloaded and run locally on PCs, Macintosh, or Unix computers. The protocols in this unit discuss how to use ClustalX and ClustalW to construct an alignment, and create profile alignments by merging existing alignments.
Clustal程序被广泛用于对核苷酸或氨基酸序列进行自动多重比对。最广为人知的版本是ClustalW，它使用一个简单的文本菜单系统，几乎可以移植到所有计算机系统。ClustalX具有图形用户界面以及一些强大的图形实用工具，有助于比对结果的解读，是交互式使用的首选版本。用户可以通过网络从多个站点远程运行Clustal，也可以下载这些程序并在个人电脑、苹果电脑或Unix计算机上本地运行。本单元中的方案讨论了如何使用ClustalX和ClustalW构建比对，并通过合并现有比对来创建概要比对。
