Hou S T, Wang C C, Chu M L
Department of Pediatrics, Tri-Service General Hospital, Taipei City, Taiwan, Republic of China.
Infect Control Hosp Epidemiol. 1997 Nov;18(11):769-71. doi: 10.1086/647534.
Between 1987 and 1989, two sequential outbreaks of nosocomial infection caused by Enterobacter cloacae occur-red in the pediatric intensive-care unit of a tertiary-care teaching hospital. Seventeen strains retrieved from the outbreaks and two control strains identified in other wards were typed by ribotyping and random amplification of polymorphic DNA (RAPD). The results indicated that the genomic pattern of strains identified between the first and second outbreaks was different. We conclude that both ribotyping and RAPD are highly discriminatory and reproducible methods for typing E cloacae. RAPD seems to be more efficacious and cost-effective.
1987年至1989年间，一家三级护理教学医院的儿科重症监护病房发生了两起由阴沟肠杆菌引起的医院感染连续暴发。从暴发中分离出的17株菌株以及在其他病房鉴定出的2株对照菌株通过核糖体分型和随机扩增多态性DNA（RAPD）进行分型。结果表明，第一次和第二次暴发之间鉴定出的菌株基因组模式不同。我们得出结论，核糖体分型和RAPD都是用于阴沟肠杆菌分型的高度鉴别性和可重复性方法。RAPD似乎更有效且更具成本效益。
