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用比色法和酶法测定淀粉酶/肌酐的肾清除率。

Determination of renal clearances of amylase/creatinine with chromogenic and enzymatic methods.

作者信息

Hohenwallner W, Wimmer E, Sommer R

出版信息

Clin Biochem. 1979 Dec;12(6):270-1. doi: 10.1016/s0009-9120(79)80125-3.

DOI:10.1016/s0009-9120(79)80125-3
PMID:93990
Abstract

Urinary amylase was estimated by chromogenic (amylochrome Roche) as well as enzymatic methods (SKI and Beckman: substrate starch and substrate maltotetraose respectively). Random and timed urines (24 hour collections) were analysed. Clearances of amylase gave different results dependent upon the amylase-test used and the glomerular filtration rate. Correlation between chromogenic and enzymatic methods (starch as substrate) was poor. The ratio of amylase and creatinine clearance was used to test different methods. Reference values for this ratio for the amylochrome method (N = 106) were 2.85 +/- 0.99% and for the Beckman-DS method (N = 60) 2.82 +/- 0.87%.

摘要

采用显色法(淀粉比色法,罗氏试剂)和酶法(SKI法和贝克曼法:分别以淀粉和麦芽四糖为底物)对尿淀粉酶进行测定。分析了随机尿和定时尿(24小时收集)。根据所使用的淀粉酶检测方法和肾小球滤过率的不同,淀粉酶清除率得出不同结果。显色法和酶法(以淀粉为底物)之间的相关性较差。淀粉酶与肌酐清除率的比值用于检验不同方法。淀粉比色法(N = 106)该比值的参考值为2.85±0.99%,贝克曼-DS法(N = 60)为2.82±0.87%。

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