Chua K S, Tan I K, Vengadiswaran R, Peiris J T
Ann Acad Med Singap. 1979 Apr;8(2):187-92.
This paper describes an investigation carried out to select a reagent kit for amylase assay to replace the Wohlgemuth method currently in use in our laboratory. The following four commercial kits were assessed: Amylochrome, Phadebas, DyAmyl-L and Amylotube. The results showed that the first three kits which employed chromogenic substrates gave better reproducibility than the Amylotube kit which used plain starch substrate and the starch-iodine reaction. Results obtained with the former methods correlated well with one another but poorly with results obtained with the latter method. Various considerations given for the recommended use of the Amylochrome method are discussed.
本文描述了一项调查，旨在选择一种淀粉酶检测试剂盒以取代我们实验室目前使用的沃格穆特法。对以下四种商用试剂盒进行了评估：淀粉比色法试剂盒、淀粉试纸法试剂盒、DyAmyl-L试剂盒和淀粉试管法试剂盒。结果表明，前三种使用显色底物的试剂盒比使用普通淀粉底物和淀粉-碘反应的淀粉试管法试剂盒具有更好的重复性。前几种方法得到的结果相互之间相关性良好，但与后一种方法得到的结果相关性较差。文中讨论了推荐使用淀粉比色法的各种考虑因素。
