从培养物和血液中提取真菌病原体DNA的不同方法比较
Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood.
作者信息
Löffler J, Hebart H, Schumacher U, Reitze H, Einsele H
机构信息
Abt. Innere Medizin II, Medizinische Klinik und Poliklinik, Tübingen, Germany.
出版信息
J Clin Microbiol. 1997 Dec;35(12):3311-2. doi: 10.1128/jcm.35.12.3311-3312.1997.
Abstract
Five commercially available extraction kits and an in-house DNA extraction method for the release of DNA from Candida albicans and Aspergillus niger cells were assessed for sensitivity, purity, duration, and cost. All commercially available kits helped to shorten the duration of DNA extraction. However, the sensitivity varied from 1 to 1,000 fungal cells/ml and costs varied from $0.10 to 2.30. The QIAmp Tissue kit was the commercially available assay that yielded the same sensitivity and purity of fungal DNA release as the in-house protocol but was the most expensive method. In comparing these two extraction protocols, a 99% concordance of PCR results for 125 blood samples analyzed could be demonstrated.
摘要
对五种市售提取试剂盒以及一种用于从白色念珠菌和黑曲霉细胞中释放DNA的内部DNA提取方法进行了灵敏度、纯度、提取时间和成本方面的评估。所有市售试剂盒都有助于缩短DNA提取时间。然而，灵敏度在1至1000个真菌细胞/毫升之间变化，成本在0.10美元至2.30美元之间变化。QIAmp组织试剂盒是市售检测方法中，在真菌DNA释放方面产生与内部方案相同灵敏度和纯度的试剂盒，但它是最昂贵的方法。在比较这两种提取方案时，对于所分析的125份血样，可证明PCR结果的一致性为99%。
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