Bergmann J S, Woods G L
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0740, USA.
J Clin Microbiol. 1997 Dec;35(12):3325-7. doi: 10.1128/jcm.35.12.3325-3327.1997.
The reliability of mycobacterial growth indicator tubes (MGIT) for testing susceptibility of Mycobacterium tuberculosis to ethambutol and streptomycin was evaluated by comparing MGIT results to those obtained by the radiometric BACTEC TB system and the method of proportion. The method of proportion was considered the reference method. To resolve discrepancies, all three testing methods were repeated. For the 74 isolates evaluated, initial ethambutol results agreed by all three methods for 64 (86.5%) of them; 58 were susceptible and 6 were resistant. MGIT and method-of-proportion results agreed for 67 isolates, and BACTEC results agreed with method-of-proportion results for 71 (P = 0.096). Initial streptomycin results obtained by all three methods agreed for 69 (93.2%) isolates: 55 were susceptible and 14 were resistant. MGIT and method-of-proportion results were concordant for 69 isolates, and BACTEC and method-of-proportion results agreed for 73 (P = 0.049). The mean times to MGIT results were 5.58 +/- 0.10 days (range, 3 to 9 days) for ethambutol and 5.47 +/- 0.11 days (range, 3 to 9 days) for streptomycin, compared to a mean of 7.41 +/- 0.20 days (range, 4 to 12 days) for both drugs with the BACTEC system (P < 0.001).
通过将分枝杆菌生长指示管（MGIT）检测结核分枝杆菌对乙胺丁醇和链霉素敏感性的结果与放射计量法BACTEC TB系统及比例法所获结果进行比较，评估了MGIT的可靠性。比例法被视为参考方法。为解决差异问题，对所有三种检测方法都进行了重复检测。对于所评估的74株分离菌，三种方法得出的初始乙胺丁醇检测结果在64株（86.5%）中一致；其中58株敏感，6株耐药。MGIT与比例法的结果在67株分离菌中一致，BACTEC的结果与比例法的结果在71株中一致（P = 0.096）。三种方法得出的初始链霉素检测结果在69株（93.2%）分离菌中一致：55株敏感，14株耐药。MGIT与比例法的结果在69株分离菌中相符，BACTEC与比例法的结果在73株中一致（P = 0.049）。MGIT得出乙胺丁醇结果的平均时间为5.58±0.10天（范围为3至9天），链霉素为5.47±0.11天（范围为3至9天），相比之下，BACTEC系统检测这两种药物的平均时间为7.41±0.20天（范围为4至12天）（P < 0.001）。
