Rusconi S, Berlusconi A, Papagno L, Colombo M C, De Maddalena C, Riva A, Bagnarelli P, Balotta C, Galli M
Istituto di Malattie Infettive, Università di Milano, Ospedale Luigi Sacco, Italy.
Clin Immunol Immunopathol. 1997 Dec;85(3):320-3. doi: 10.1006/clin.1997.4470.
With the aim of evaluating the specific pattern of in vitro antibody production (IVAP) in human immunodeficiency virus type 1 (HIV-1)-infected long-term non-progressors (LTNPs), we tested 20 subjects who had remained asymptomatic for more than 8 years with a CD4+ cell count higher than 500/microliter and 59 patients at different stages of HIV-1 infection as controls. In cell cultures, IVAP was detected in 14 out of 20 LTNPs (70%), in 5 out of 6 recent seroconverters (83%), and in all the other control patients. Anti-p24 antibody production was significantly lower in LTNPs than in asymptomatic patients with a more recent infection. Recent seroconverters and patients with AIDS did not produce anti-p24 antibodies (P = 0.02). Anti-gp160 antibodies were produced by peripheral blood mononuclear cells from LTNPs in 12/20 cases. CD4+ cell count was significantly higher in IVAP-negative than in IVAP-positive LTNPs (P = 0.013), while the viral load was not significantly different. Specific anti-HIV-1 antibody production did not seem to be a correlate of long-term nonprogression.
为了评估1型人类免疫缺陷病毒（HIV-1）感染的长期不进展者（LTNP）的体外抗体产生（IVAP）的特定模式，我们检测了20名无症状超过8年且CD4 +细胞计数高于500/微升的受试者，以及59名处于HIV-1感染不同阶段的患者作为对照。在细胞培养中，20名LTNP中有14名（70%）检测到IVAP，6名近期血清转化者中有5名（83%）检测到IVAP，所有其他对照患者也检测到IVAP。LTNP中抗p24抗体的产生明显低于近期感染的无症状患者。近期血清转化者和艾滋病患者不产生抗p24抗体（P = 0.02）。12/20的LTNP外周血单个核细胞产生抗gp160抗体。IVAP阴性的LTNP的CD4 +细胞计数明显高于IVAP阳性的LTNP（P = 0.013），而病毒载量无明显差异。特异性抗HIV-1抗体的产生似乎与长期不进展无关。
