Montefiori D C, Pantaleo G, Fink L M, Zhou J T, Zhou J Y, Bilska M, Miralles G D, Fauci A S
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
J Infect Dis. 1996 Jan;173(1):60-7. doi: 10.1093/infdis/173.1.60.
Serum antibodies from human immunodeficiency virus type 1 (HIV-1)-infected long-term non-progressors (LTNPs) and non-LTNPs were evaluated for virus neutralization and infection enhancement in vitro. Sera from LTNPs had higher average titers of neutralizing antibodies to HIV-1 strains IIIB and MN and more frequently neutralized primary isolates from progressors (14.9% vs. 1.3%, P = .002). Replication-competent HIV-1 was isolated from peripheral blood mononuclear cells and lymph nodes of 3 LTNPs. All viruses from LTNPs had a non-syncytium-inducing phenotype, were resistant to neutralization by autologous serum obtained at the time of virus isolation, and showed little evidence of a heightened sensitivity to neutralization by heterologous sera. Complement-mediated, antibody-dependent enhancement (C'-ADE) of HIV-1IIIB and primary isolates was equally prevalent for sera from LTNPs and non-LTNPs. Results indicate that LTNPs produce vigorous serum antibody responses and that long-term nonprogression is not associated with homologous neutralization or the absence of C'-ADE.
对来自人类免疫缺陷病毒1型（HIV-1）感染的长期不进展者（LTNP）和非LTNP的血清抗体进行体外病毒中和及感染增强评估。LTNP的血清对HIV-1毒株IIIB和MN具有更高的平均中和抗体滴度，且更频繁地中和来自进展者的原始分离株（14.9%对1.3%，P = 0.002）。从3名LTNP的外周血单核细胞和淋巴结中分离出具有复制能力的HIV-1。来自LTNP的所有病毒均具有非合胞体诱导表型，对病毒分离时获得的自体血清的中和作用具有抗性，并且几乎没有证据表明对异源血清的中和作用高度敏感。HIV-1IIIB和原始分离株的补体介导的抗体依赖性增强（C'-ADE）在LTNP和非LTNP的血清中同样普遍。结果表明，LTNP产生强烈的血清抗体反应，并且长期不进展与同源中和或不存在C'-ADE无关。
