Peeters B, Bienkowska-Szewczyk K, Hulst M, Gielkens A, Kimman T
Institute for Animal Science and Health (ID-DLO), Department of Virology, Lelystad, The Netherlands.
J Gen Virol. 1997 Dec;78 ( Pt 12):3311-5. doi: 10.1099/0022-1317-78-12-3311.
Envelope glycoprotein D (gD) of pseudorabies virus (PRV) is essential for penetration but is not required for cell-to-cell spread. When animals are inoculated with a phenotypically complemented PRV gD mutant, the virus is able to spread locally by means of direct cell-to-cell transmission, but progeny virions released by infected cells are non-infectious because they lack gD. Therefore, the virus cannot be transmitted from inoculated animals to other animals. This property makes a PRV gD mutant an attractive candidate as a safe vaccine vector. To examine whether a self-restricted, non-transmissible PRV mutant can be used as a biologically safe vaccine vector, a gD/gE-negative PRV recombinant virus which expresses envelope glycoprotein E2 of classical swine fever virus was constructed. Vaccination of pigs showed that the recombinant virus was able to protect pigs against both Aujeszky's disease and classical swine fever.
伪狂犬病病毒（PRV）的包膜糖蛋白D（gD）对于病毒穿透至关重要，但对于细胞间传播并非必需。当用表型互补的PRV gD突变体接种动物时，病毒能够通过直接的细胞间传播在局部扩散，但受感染细胞释放的子代病毒粒子由于缺乏gD而无感染性。因此，该病毒无法从接种动物传播至其他动物。这一特性使得PRV gD突变体成为一种有吸引力的安全疫苗载体候选物。为了检验自我限制、不可传播的PRV突变体是否可用作生物安全疫苗载体，构建了一种表达经典猪瘟病毒包膜糖蛋白E2的gD/gE阴性PRV重组病毒。给猪接种疫苗表明，该重组病毒能够保护猪抵抗奥耶斯基氏病和经典猪瘟。
