Hinchcliff K W, Reinhart G A, Burr J R, Schreier C J, Swenson R A
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210-1089, USA.
Am J Vet Res. 1997 Dec;58(12):1457-62.
To measure energy expenditures of Alaskan sled dogs at rest and during racing under frigid conditions, using the doubly labeled water (DLW) technique.
18 fit Alaskan sled dogs.
Energy expenditure was measured in 9 dogs during a 490-km sled dog race by use of the DLW technique, whereby dogs were administered water enriched with nonradioactive isotopes of hydrogen and oxygen. Energy intake was determined by dietary analysis. Changes in background abundance of the isotopes 2H and 18O were monitored in 5 dogs that did not receive isotope-enriched water.
Dogs completed the 490-km race at an average speed of 7 km/h at ambient temperature of -35 to -10 C. Total energy expenditure, measured by the DLW technique, was 47,100 +/- 5,900 kJ/d (4,400 +/- 400 kJ.kg-0.75/d), and metabolizable energy intake was 44,600 kJ/d (4,100 kJ.kg-0.75/d) during the 70-hour race.
The sustained metabolic rate for these sled dogs during racing was extraordinarily high for a large mammal. This study validated use of the DLW technique in dogs with exceptionally high energy expenditure associated with prolonged exercise in the cold.
使用双标记水（DLW）技术测量阿拉斯加雪橇犬在寒冷条件下休息和比赛期间的能量消耗。
18只健康的阿拉斯加雪橇犬。
在一场490公里的雪橇犬比赛中，对9只犬使用DLW技术测量能量消耗，即给犬饮用富含氢和氧的非放射性同位素的水。通过饮食分析确定能量摄入。对5只未饮用富含同位素水的犬监测同位素2H和18O的背景丰度变化。
犬在环境温度为-35至-10摄氏度的条件下，以平均7公里/小时的速度完成了490公里的比赛。在为期70小时的比赛中，通过DLW技术测得的总能量消耗为47100±5900千焦/天（4400±400千焦·千克-0.75/天），可代谢能量摄入为44600千焦/天（4100千焦·千克-0.75/天）。
这些雪橇犬在比赛期间的持续代谢率对于大型哺乳动物来说异常高。本研究验证了DLW技术在与寒冷环境中长时间运动相关的能量消耗极高的犬中的应用。
