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Synthesis and enzymatic stability of phosphodiester-linked peptide-oligonucleotide hybrids.

作者信息

Robles J, Maseda M, Beltrán M, Concernau M, Pedroso E, Grandas A

机构信息

Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Spain.

出版信息

Bioconjug Chem. 1997 Nov-Dec;8(6):785-8. doi: 10.1021/bc970051u.

DOI:10.1021/bc970051u
PMID:9404649
Abstract

Nucleopeptides Ac-Tyr(p3' dACGT)-Ala-Phe-Gly-NH2, Ac-Thr(p3'dACGT)-Ala-Phe-Gly-OH, Ac-Ser(p3'dACGT)-Ala-Phe-Gly-OH, and Phac-Hse(p3'dACGT)-Ala-Phe-Gly-OH, in which the 3'-end of a tetradeoxyribonucleotide is linked by a phosphodiester bond to a hydroxylated amino acid, were synthesized using a stepwise solid-phase methodology to study the influence of the linking amino acid on their stability to 3'-exonucleases. HPLC analysis of the reaction crudes after treatment of each nucleopeptide with snake venom phosphodiesterase showed that the lability of the amino acid-nucleoside linkage increases in the order Thr < Ser < Hse < Tyr.

摘要

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