Anderson K A, Tschirgi M L
University of Idaho, Department of Food Science and Toxicology, Moscow 83844-2201, USA.
Biol Trace Elem Res. 1997 Oct-Nov;60(1-2):27-37. doi: 10.1007/BF02783307.
A benign-by-design method for the determination of boron (B) in microsamples of biological tissues was developed. This is a simple, automated, microdigestion method. Use of reagents and generation of waste are minimized, and the use of toxic/hazardous reagents is eliminated as compared to currently available B methodology. Microsamples are accommodated by the method; 100-400 mg samples were used in this study. B is determined by inductively coupled plasma atomic emission spectrometry (ICPAES) at 249.678 nm. The instrument detection limit for B is 0.01 microgram/mL. Interference studies have been investigated for 21 common elements. Over 250 analyses of standard reference materials were analyzed during the study duration. Recoveries for a series of biological tissues, both plant and animal, ranged from 82-104%.
开发了一种用于测定生物组织微量样品中硼（B）的设计安全的良性方法。这是一种简单的自动化微量消解方法。与目前可用的硼测定方法相比，试剂的使用和废物的产生被最小化，并且消除了有毒/有害试剂的使用。该方法适用于微量样品；本研究中使用了100 - 400毫克的样品。通过电感耦合等离子体原子发射光谱法（ICPAES）在249.678纳米处测定硼。硼的仪器检测限为0.01微克/毫升。对21种常见元素进行了干扰研究。在研究期间对超过250份标准参考物质进行了分析。一系列植物和动物生物组织的回收率在82% - 104%之间。
