Nishizawa T, Okamoto H, Konishi K, Yoshizawa H, Miyakawa Y, Mayumi M
Immunology Division, Jichi Medical School, Tochigi-Ken, 329-04, Japan.
Biochem Biophys Res Commun. 1997 Dec 8;241(1):92-7. doi: 10.1006/bbrc.1997.7765.
By means of representational difference analysis, a viral clone (N22) of 500 nucleotides was isolated from serum of a patient (TT) with posttransfusion hepatitis of unknown etiology. The N22 clone showed a poor homology to any reported sequences. Oligonucleotide primers were deduced from the N22 sequence for detecting it by polymerase chain reaction. N22 sequence in serum banded at a sucrose density of 1.26 g/cm3, indicating its association with a viral particle which was designated TT virus (TTV). Since nucleic acids of TTV were sensitive to DNase I, it would be a DNA virus. TTV DNA was detected in sera from three of the five patients with posttransfusion non-A to G hepatitis, including the index case (TT). TTV DNA titers closely correlated with aminotransferase levels in the three patients. These results indicate that TTV would be a novel DNA virus with a possible capacity to induce posttransfusion non-A to G hepatitis.
通过代表性差异分析，从一名病因不明的输血后肝炎患者（TT）的血清中分离出一个500个核苷酸的病毒克隆（N22）。N22克隆与任何已报道的序列同源性较差。根据N22序列推导寡核苷酸引物，用于通过聚合酶链反应检测它。血清中的N22序列在蔗糖密度为1.26 g/cm3处出现条带，表明它与一种病毒颗粒相关，该病毒颗粒被命名为TT病毒（TTV）。由于TTV的核酸对DNA酶I敏感，它可能是一种DNA病毒。在五名输血后非甲至庚型肝炎患者中的三名患者的血清中检测到TTV DNA，包括首例患者（TT）。这三名患者的TTV DNA滴度与转氨酶水平密切相关。这些结果表明，TTV可能是一种新型DNA病毒，有可能导致输血后非甲至庚型肝炎。
