成人期华支睾吸虫体细胞抗原与排泄分泌抗原在酶联免疫吸附试验中用于人体异盘吸虫感染血清学诊断的比较

Comparison of adult somatic and excretory-secretory antigens in enzyme-linked immunosorbent assay for serodiagnosis of human infection with Paragonimus heterotremus.

作者信息

Maleewong W, Pariyanonda S, Wongkham C, Intapan P, Daenseegaew W, Morakote N

机构信息

Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand.

出版信息

Trans R Soc Trop Med Hyg. 1990 Nov-Dec;84(6):840-1. doi: 10.1016/0035-9203(90)90102-k.

DOI:10.1016/0035-9203(90)90102-k

PMID:2096519

Abstract

Adult somatic antigen extract of Paragonimus heterotremus was compared with excretory-secretory (ES) antigen in an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of human paragonimiasis. The absorbence values in ELISA using the adult somatic antigen were not significantly different from the values obtained using ES antigens (P greater than 0.05). The sensitivity of the ELISA using either antigen was 100%, but the specificity was 96% with somatic and 98% with ES antigens due to a cross reaction with fascioliasis sera. It appears that both somatic and ES antigens are effective antigens for use in serodiagnosis of human paragonimiasis heterotremus.

摘要

在用于人体肺吸虫病血清学诊断的酶联免疫吸附测定（ELISA）中，对异盘肺吸虫成虫体抗原与排泄分泌（ES）抗原进行了比较。使用成虫体抗原的ELISA中的吸光度值与使用ES抗原获得的值无显著差异（P大于0.05）。使用任何一种抗原的ELISA的敏感性均为100%，但由于与肝片吸虫病血清存在交叉反应，成虫体抗原的特异性为96%，ES抗原的特异性为98%。看来成虫体抗原和ES抗原都是用于人体异盘肺吸虫病血清学诊断的有效抗原。